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Fig. 6 Immunohistochemical detection of the distribution of YAP and GLI2? in TAA-induced liver fibrosis. A Representative fluorescence microscopy of the distributions of YAP and GLI2? in liver sections using the indicated antibodies: anti-YAP (red), anti-GLI2? (green) and nuclei were stained with 4?, 6-diamidino-2-phenylindole DAPI (blue). The corresponding overlay of YAP and GLI2? is shown in panel (YAP + GLI2?) and the corresponding overlays of the three signals is shown in panel (merge). Scale bar = 50 µm. B Quantification of relative fluorescence intensity of YAP and GLI2? in control and TAA-induced liver fibrosis. C Percent of YAP/GLI2? nuclear colocalization in control and TAA-induced liver fibrosis. The fluorescence intensity of each section was measured by ImageJ. *p < 0.05, n = 5, means ± SD