Fig. 6 Immunohistochemical detection of the distribution of YAP and GLI2α in TAA-induced liver fibrosis. A Representative fluorescence microscopy of the distributions of YAP and GLI2α in liver sections using the indicated antibodies: anti-YAP (red), anti-GLI2α (green) and nuclei were stained with 4′, 6-diamidino-2-phenylindole DAPI (blue). The corresponding overlay of YAP and GLI2α is shown in panel (YAP + GLI2α) and the corresponding overlays of the three signals is shown in panel (merge). Scale bar = 50 µm. B Quantification of relative fluorescence intensity of YAP and GLI2α in control and TAA-induced liver fibrosis. C Percent of YAP/GLI2α nuclear colocalization in control and TAA-induced liver fibrosis. The fluorescence intensity of each section was measured by ImageJ. *p < 0.05, n = 5, means ± SD
Image
Figure Caption
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ J. Cell Commun. Signal.