Fig. 1.

Fig. 1.

Structure of the human MDM2/p53TAD complex. a) Schematic domain architecture of MDM2 and p53. b) Crystal structure of the human complex between the SWIB domain of MDM2 (gold) and a peptide corresponding to the conserved binding motif in p53TAD (blue) (PDBid: 1ycr) (Kussie et al. 1996). Arrows highlight the conserved hydrophobic triad where the residues are shown as sticks. c) Sequence alignment of reconstructed and extant canonical MDM2-binding motifs of p53TAD. To facilitate comparison, the site numbering used here follows the human p53TAD-binding motif, from Ser₁₅ to Pro₂₇. The previously reconstructed maximum likelihood sequence for the most recent common ancestor of bony fishes and tetrapods (Mihalič et al. 2023) is indicated in the alignment as “fish versus tetrapods.”