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Figure 4

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ZDB-IMAGE-240226-147
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Figures for Zhou et al., 2024
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Figure 4

Reactive oxygen species can affect neutrophil migration and immune function (ad) Pattern diagrams showing the caudal fin damage model and otic vesicle inflammation model using zebrafish from the Tg(lyz:EGFP) strain. Fluorescence images showing migrating neutrophils. The white rectangles indicate the counting area (scale bar: 200 μm). H2O2 treatment significantly increased neutrophil recruitment to the injury site, and metformin mitigated this effect (n = 20). (eh) Q-PCR results showing that metformin treatment decreased the upregulation of the inflammatory genes il-1β, il-6, cxcl8a, and tnf-α induced by H2O2 in juvenile zebrafish. (il) Q-PCR results showed that metformin treatment decreased the upregulation of the inflammatory genes il-1β, il-6, cxcl8a, and tnf-α induced by H2O2 in murine macrophages. (**** p  <  0.0001, *** p  <  0.001, ** p  <  0.01, * p  <  0.05, “ns”, no statistical difference).

Acknowledgments
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