Reactive oxygen species can affect neutrophil migration and immune function (a–d) Pattern diagrams showing the caudal fin damage model and otic vesicle inflammation model using zebrafish from the Tg(lyz:EGFP) strain. Fluorescence images showing migrating neutrophils. The white rectangles indicate the counting area (scale bar: 200 μm). H2O2 treatment significantly increased neutrophil recruitment to the injury site, and metformin mitigated this effect (n = 20). (e–h) Q-PCR results showing that metformin treatment decreased the upregulation of the inflammatory genes il-1β, il-6, cxcl8a, and tnf-α induced by H2O2 in juvenile zebrafish. (i–l) Q-PCR results showed that metformin treatment decreased the upregulation of the inflammatory genes il-1β, il-6, cxcl8a, and tnf-α induced by H2O2 in murine macrophages. (**** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, “ns”, no statistical difference).
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ Antioxidants (Basel)
Your Input Welcome
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if
additional information is required.
Oops. Something went wrong. Please try again later.