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Figure 2

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ZDB-IMAGE-240226-145
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Figures for Zhou et al., 2024
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Figure 2

Metformin reduces the recruitment of neutrophils to inflammatory sites. (a,c) Pattern diagrams showing the caudal fin damage model and otic vesicle inflammation model in zebrafish infected with the Tg(lyz:EGFP) strain. Fluorescence images showing migrating neutrophils. The white rectangles indicate the counting area (scale bar: 200 μm). (b,d) Statistical analysis showed that the number of neutrophils recruited to the injury site was significantly lower in the larvae-fed metformin (n = 20). (eh) Q-PCR analysis showed that the expression levels of the inflammation-related genes il-1β, il-6, cxcl8a, and tnf-α were significantly downregulated after metformin treatment in juvenile fish. (il) Q-PCR analysis showed that the expression levels of the inflammation-related genes il-1β, il-6, cxcl8a, and tnf-α in murine macrophages under metformin conditions were significantly downregulated. (**** p < 0.0001, *** p < 0.001, ** p  <  0.01, * p  <  0.05, “ns”, no statistical difference).

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