|
Fig. 5 Zebrafish cell division cycle 10 (Cdc10)-dependent transcript 1 (cdt1)-201 mutant is viable but suffers from growth retardation and infertile. (a) Schematic diagram depicting generation of the cdt1-201 mutant (mu) with guide RNA (gRNA) targeting of exon 2 of cdt1-201. (b) Expression of molecular markers from the three germ layers in 5 dpf wild type (WT) siblings (left panel) and mutant (mu) (right panel). Fraction represents number of observed phenotypes/total embryos scored. (c) Table shows the actual numbers of viable siblings and mutants (mu) scored from 5 dpf to 3 mpf. Bar chart shows the survival scores (%) of mutant from 5 dpf to 3 mpf. (d, e) Comparison of 8 mpf WT and mutant (males) in overall body size and size of various major organs including liver, heart, spleen, intestine, testis, and brain. Scale is in cm. Sample number in each group is three. (f) The mean area of each organ is tabulated into a bar graph. Standard deviation is not calculated due to too small sample size (n=3, for each organ). dpf: days post fertilization; mpf: months post fertilization.