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Fig. 4 Transcript cell division cycle 10 (Cdc10)-dependent transcript 1 (cdt1) -202 is barely detectable in wild type (WT) but increases in cdt1-201 mutant. (a) Expression levels of cdt1-201 and cdt1-202 transcripts in WT vs. mutant (mu) fries, obtained by RNA sequencing (RNA-seq). The transcripts per million (TPM) values derived from RNA-seq data are shown (upper panel). (b) Alignment of cdt1-201 and cdt1-202 reverse transcription-polymerase chain reaction (RT-PCR) products amplified by primers on exon 1 and exon 3. (c) Expression levels of cdt1-201 and cdt1-202 transcripts in WT vs. mu embryos or fries, WT testis, and WT ovary, obtained by RNA reverse transcription followed by single-colony PCR. Positions of primers are indicated. (d) Expression levels of cdt1-201 and cdt1-202 transcripts in WT organs, obtained by RNA-seq. The TPM values derived from RNA-seq data are shown in tables (upper panel). Data are expressed as mean±standard deviation (SD), n=3 to 10 based on specific tissue size (e.g., three fishes vs. ten hearts). * P<0.05, ** P<0.01, two-tailed t-test. dpf: days post fertilization; mpf: months post fertilization.