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Fig. 2 Neutrophils infiltrate into livers in atp7b-/- zebrafish indicated by mpx-positive neutrophils. (A) Representative images of mpx+/atp7b+/+ and mpx+/atp7b-/- fish after 0, 6, 12, 18, and 24 hours of Cu treatment. The livers are outlined in blue dashed lines. hpt, hours post-treatment. Scale bar: 200 ?m. (B) Neutrophil count, (C) liver size, and (D) neutrophil density in wild-type and mutant fish livers. Data are the means ± SEM, n = 10 fish /group. ?P < .05, ??P < .01.
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