FIGURE 5

FIGURE 5

Deficiencies of reticulospinal neurons in angpt1^–/– and itgb1b^–/– larval hindbrain. Expression of krox20 mRNA done by WISH shown in (A) lateral view and (C) dorsal view of 3-dpf angpt1^+/+ and (G) lateral view and (I) dorsal view of 4-dpf itgb1b^+/+ larvae. Fainter and misexpression patterns of krox20 were found in (B) lateral view and (D) dorsal view of 3-dpf angpt1^–/– brain and in (H) lateral view and (J) dorsal view of 4-dpf itgb1b^–/– brain. Retrograde labeling of 4-dpf genotyped larvae reveals the deficiencies of reticulospinal neurons in (F)angpt1^–/– and (L)itgb1b^–/– brains compared with their (E)angpt1^+/+ and (K)itgb1b^+/+ siblings. N = 5 per genotyped group in WISH and retrograde labeled analysis. Arrows indicate the abnormality of krox20 expression patterns in the angpt1^–/– and itgb1b^–/– hindbrain and lateral line nerves. Scale bars are 200 μm.