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Fig. 11 Dil-labeled MCF-7 cells (red) and FITC-labeled D-BSA NPs (green) injected zebrafish larvae. Images were taken at the injection day (0 dpni) (A) and 1 day post-injection (1 dpni) (A′). (B) CTCs at the day of D-BSA NPs and DOX-loaded D-BSA NP injections (0 dpni) and 1 day post-NP injections (1 dpni) were counted in 5 individual larvae and average cell numbers are normalized with their 0 dpni values. Upon 1 day treatment with DOX-loaded D-BSA NPs, number of CTCs decreased by 30% with p ≤ 0.001 significance (***), whereas the CTC numbers of D-BSA NP-treated larvae did not change significantly p > 0.05). Scale = 500 μm