PUBLICATION
Dopamine-Conjugated Bovine Serum Albumin Nanoparticles Containing pH-Responsive Catechol-V(III) Coordination for In Vitro and In Vivo Drug Delivery
- Authors
- Argitekin, E., Ersoz-Gulseven, E., Cakan-Akdogan, G., Akdogan, Y.
- ID
- ZDB-PUB-230715-62
- Date
- 2023
- Source
- Biomacromolecules 24(8): 3603-3618 (Journal)
- Registered Authors
- Cakan-Akdogan, Gülcin
- Keywords
- none
- MeSH Terms
-
- Animals
- Catechols/pharmacology
- Dopamine
- Doxorubicin/chemistry
- Doxorubicin/pharmacology
- Drug Carriers/chemistry
- Drug Delivery Systems
- Drug Liberation
- Ferric Compounds
- HEK293 Cells
- Humans
- Hydrogen-Ion Concentration
- Nanoparticles*/chemistry
- Serum Albumin, Bovine*/chemistry
- Zebrafish
- PubMed
- 37450837 Full text @ Biomacromolecules
Citation
Argitekin, E., Ersoz-Gulseven, E., Cakan-Akdogan, G., Akdogan, Y. (2023) Dopamine-Conjugated Bovine Serum Albumin Nanoparticles Containing pH-Responsive Catechol-V(III) Coordination for In Vitro and In Vivo Drug Delivery. Biomacromolecules. 24(8):3603-3618.
Abstract
V(III) instead of commonly used Fe(III) provided a rich tris-catechol-metal coordination at pH 7.4, which is important for slow drug release at physiological pH. Bovine serum albumin (BSA) functionalized with catechol-containing dopamine (D) and cross-linked using tris-catechol-V(III) coordination yielded pH-responsive compact D-BSA NPs (253 nm). However, conversion to bis- and/or mono-catechol-V(III) complexes in an acidic medium resulted in degradation of NPs and rapid release of doxorubicin (DOX). It was shown that D-BSA NPs entered cancerous MCF-7 cells (66%) more efficiently than non-cancerous HEK293T (33%) in 3 h. Also, DOX-loaded NPs reduced cell viability of MCF-7 by 75% and induced apoptosis in a majority of cells after 24 h. Biodegradability and lack of hemolytic activity were shown in vitro, whereas a lack of toxicity was shown in histological sections of zebrafish. Furthermore, 30% of circulating tumor cells in vasculature in 24 h were killed by DOX-loaded NPs shown with the zebrafish CTC xenograft model.
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Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
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Mapping