Figure 2

Figure 2

4-PBA inhibits development of lens cell masses in plod3 mutants: (A,C) Live 4 dpf plod3^vu222 mutant larvae, untreated (A) or treated with 4-PBA from 26 hpf (C). Arrows point at lenses. (B,D) Histological sections of eyes from 4 dpf plod3 mutant larvae, untreated (B) or treated with 4-PBA from 26 hpf (D). Arrow in (B) points at the abnormal cell mass and in (D) at the ALE that remains a monolayer. (E) Statistical analysis of the rescue with 4-PBA. Outside, normal and inside categories refer to the location of the lens relative to the normal location. N depicts the number of eyes analyzed. (F) Time windows of treatment with 4-PBA are represented by bars. Green bars show time windows with statistically significant rescue and red bar represents a time window in which no statistically significant rescue was observed. Numbers on bars are p-values. L, lens. Scale bars are 100 µm (A) or 20 µm (B).