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Fig. 2
The relationship between temperature and the expression levels of MuRF1 and atrogin-1 in zebrafish muscle tissues. The relative expression levels were normalized using EF-1α as a reference and presented as a ratio to the initial water temperature of 25 °C (A; n = 4) and the water temperature of 25 °C (B; n = 8). The expression levels are presented as means (A; n = 4) and means ± standard deviations (B; 25, 20, and 15 °C: n = 8; 10 °C: n = 24).
A. Asterisks indicate significant differences compared to the relative expression level at the initial water temperature of 25 °C (vs 25 d3; *: p < 0.05, **: p < 0.01, ***: p < 0.001).
B. In the post-hoc test, asterisks indicate statistical significance in comparisons between expression levels at different temperatures (*: p < 0.05, **: p < 0.01, ***: p < 0.001).