Figure 17

Figure 17

(a) The 3D images of zebrafish trunk vessels. (b–g) and (h,i) are enlarged images of areas 1 and 2 in (a), respectively. (b–d) Fluorescence images of Tg(fli1a:EGFP) after incubation with compound 2 (40 µM) for 1 h in 48 hpf larvae. In (c), most of the compounds adhere to the surface of the blood vessels, and a few enter the blood vessels. (e–g) Fluorescence images of Tg(fli1a:EGFP) after incubation with compound 2 (40 µM) for 2 h at 48 hpf. Arrowheads in (c,f) indicate that compound 2 adheres to the surface of the blood vessels. In (f), the amount of compound absorbed into the blood vessels is greatly increased. In (h,i), compound 2 enters the CVP. Zebrafish blood vessels are characterized by green fluorescence. Scale bars, 500 µm (a), 200 µm (b,e,h), 100 µm (c,f), and 50 µm (d,g,i).