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Figure 1
Schematic diagram of gpr56 gene knockout in zebrafish. (A) Schematic diagram of sgRNA targeting used in gpr56 gene knockout. The green horizontal line represents the genomic DNA of gpr56, the green rectangle represents the exons of gpr56, red shows the target sequence, and blue shows PAM [23]. (B) Schematic diagram of three heritable mutant alleles of gpr56 generated by gene knockout and the protein sequences encoded by them. (C) Sequencing peaks of three mutant alleles of gpr56, in which A, G, C and T are shown by green, black, blue and red curves respectively.
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Full text @ Int. J. Mol. Sci.