Figure 4
ISH at 11 hpf after bmp4-induction at 8.5 hpf. (A): summary of experimental procedure: embryos were heat-shocked at 8.5 hpf and analyzed at 11 hpf; left columns wildtypes, right columns bmp4-induced;first and third column dorsal view, second and fourth column lateral view. (B–E): Expression of shha is reduced in the area of the prechordal plate (arrows) after bmp4-induction yet still present. (F–I): shhb expression is also still present after bmp4-induction but reduced in intensity (arrows). (K–N): Expression of zic2a is absent in the ANP domain (arrows) after bmp4-induction. (O–R): expression of cxcr4a in the eye field is strongly reduced after bmp4-induction. scalebars indicate 200 µm.