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Figure 2
MUC-1 and TVBF-7 ACC cells induced metastases formation in zebrafish embryos. (A) Enlargement of the embryo tail with metastasized MUC-1 cells. (B) A representative acquisition of the metastases of TVBF-7 cells in the pericardial area of the embryo is shown. Cells are labeled with a red fluorescent lipophilic dye while the embryos’ endothelium is labeled with a green fluorescent protein reporter driven by the kdrl promoter. Images were acquired at 120 hpf using a Zeiss LSM 510 META confocal laser scanning microscope at 10× magnification.
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