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Figure 3
Quantification of CyHV-2 and CyHV-3-infected cells in ZF4 monolayer over time. This data was acquired via time-lapse fluorescent microscopy (IncuCyte). Cells were cultured in a 24-well plate and infected with CyHV-2 Luc-copGFP or CyHV-3 EGFP recombinants (1.2 × 106 PFU/mL for each recombinant). At 24 hpi, cells were imaged every 2 h for 11 days. Data represent the mean ± standard errors from three replicates/wells. Data from each replicate at each timepoint represent the sum of fluorescent cells observed in nine separate locations of each well.
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