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Figure 3
Intramyocellular lipids. (A) Representative images of skeletal muscle sections in 21 dpf larvae. Nuclei stained with Hoechst (blue), actin stained with phalloidin (red), LDs stained with Bodipy665 (white). (B) LD count per fish. (C) Average LD volume per fish. (D) Percentage of LDs with a volume >2 μm3. (E) LD volume density. (F) LD volume distribution. Bars represent mean ± SEM, n = 9 pooled from three independent experiments (Kruskal–Wallis test with Dunn’s multiple comparisons correction). For all panels: #p = 0.0586, ∗p < 0.05, ∗∗p ≤ 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.
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