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Figure 2
Developmental and lifelong exposure to CPF in zebrafish ovaries promotes POA. (a) Graph represents the fertilization percentage estimated by counting the number of fertilized eggs obtained from five independent matings involving zebrafish females exposed to CPF vs. females not exposed (Vehicle). (b) Granulosa cell markers (amh) were detected by RT-qPCR. (c–e) OAGS genes (dre-mir-143, dre-mir-145, and gas5) were verified by RT-qPCR. (f,g) Representative Western blot analysis showing the level of Foxo3a/P-Foxo3a protein following CPF treatment (n = 3/group). (h) Telomere length was measured from total genomic ovaries DNA by using a qPCR. Data were obtained normalizing using tubaI for mRNA, β-actin for proteins, and U6 for miRNA). Data are mean ± s.d. with five animals per group. Significant differences are indicated with * p < 0.05; ** p < 0.01, *** p < 0.001, and **** p < 0.0001 using Student’s t-test.