Figure 3
Effects of L. gasseri on HCD-induced oxidative stress and macrophage aggregation in the liver of zebrafish larval. (A) Reactive oxygen species (ROS) staining with 2′,7′-dichlorodihydrofluorescein (DCFH-DA). Liver was outlined with yellow dot circles. (B) Quantification of ROS staining by measuring the green fluorescence density of zebrafish liver (n = 10). (C) Detection of the contents of malondialdehyde (MDA) in vivo (n = 10). (D) Infiltration of macrophages in the liver was examined using the reporter transgenic line Tg (mpx:mCherry). Macrophages were labeled with red fluorescence. Liver was indicated with blue dot circles. (E) Quantification of the number of macrophages in the liver of zebrafish (n = 10). Scale bars = 100 µm. Values were represented as mean ± SD. * p < 0.05.