Figure 6

Figure 6

Effect of PET NPs treatment on the metabolic profile of intact zebrafish embryos. Zebrafish embryos (72 hpf) were exposed to 100 ppm PET NPs for 24 h. Concentrations of metabolites relative to total creatine (tCr) are shown. For statistical analysis, one-way ANOVA with a Tukey post-hoc correction for multiple comparisons were performed using OriginPro v. 8 (Northampton, MA, USA). Values shown are the mean ± standard deviation (n = 6). The statistically significant differences in metabolites between PET NPs treated and control group obtained by ANOVA analysis are indicated by # p < 0.001, ** p < 0.01 and * p < 0.05. Abbreviations: Phe = phenylalanine; Trp = tryptophan; Tyr = tyrosine; Leu = leucine, Ile = isoleucine; Val = valine; Glu = glutamate; Gln = glutamine; Gly = glycine; Ala = alanine; Cys = cysteine; GABA = g-aminobutyric acid; GSH = glutathione; TMAO = trimethylamine N-oxide; Glc = glucose; Lac = lactate; ATP = adenosine triphosphate; NADH = nicotinamide adenine dinucleotide; m-Ins = myo-inositol; Cho = choline; GPC = glycerophosphocholine; Chol = cholesterol; FA = fatty acids.