ZFIN Image: Weeks et al., 2022, Figure 1

Image

Figure Caption

Figure 1

RNA-Seq identifies the ubiquitin proteasome system as a target of embryonic alcohol exposure.

(A) Schematic of RNA-Seq performed on 7 dpf whole-larval extracts following 0% or 1% EtOH exposure (12 hpf–5 dpf). (B) Heatmap of significantly dysregulated genes (P_adj < 0.05; n = 1,603). (C) GOrilla GSEA identifies GO components enriched in the dysregulated gene set. (D) Heatmap of genes involved in proteasome-mediated, ubiquitin-dependent protein catabolism. Following EAE, these genes were significantly upregulated relative to controls. (E) ISH for psmb1 following 0% or 1% EtOH exposure (12–96 hpf). At 96 hpf, psmb1 expression is increased in the liver (black arrow) and intestine (white star). Scale bars: 100 μm. (F) Overview of the protocol for treatment and isolation of GFP⁺ hepatocytes via FACS sorting at 120 hpf. (G) ef1α-normalized qPCR of proteasome-related genes in GFP⁺ hepatocytes sorted by FACS (*P ≤ 0.05, **P ≤ 0.01, 2-sided t test; n = 5 per column). Data represent mean ± SEM.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ JCI Insight