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FIGURE 3
Morphology and number of hair cells and support cells in Loxhd1b-knockdown and control zebrafish. (A) Reduced numbers of neuromast hair cells in Loxhd1b-morphant fish labeled by FM1-43FX at 72 and 96 hpf. The arrows point to the position of posterior lateral line neuromasts. Scale bar = 300 μm. (B,C) Immunostaining of zebrafish lateral line hair cells and support cells at 72 hpf. Hair cells are stained with myosin-7a antibody and support cells were stained with SOX2 antibody. Scale bar = 10 and 20 μm. (D–G) Statistical analysis of the hair cell clusters in control and Loxhd1b-morphan based on t-test. ***P < 0.001 and ****P < 0.0001.