Fig. 5
Fig. 5 Changes in ndrg transcript levels in response to anoxia. Real-time qPCR analysis of 24 hpf zebrafish embryos exposed to 4 h (A, grey bars) or 8 h (B, white bars) of anoxia relative to normoxic (stage-matched) controls (A, B, black bars) normalized to ef1a. (C) Plotted graphical summary of qPCR results. The y-axis in the graphs represents the relative normalized expression of each gene. All fold changes were derived using the formula, 2?(??CT), error bars represent standard error of the mean. Significance was obtained using the unpaired, two-tailed t-test with Welch?s Correction. *p < .05, **p < .01, ***p < .0005, ****p < .0001. Reactions were run in triplicate with 7?8 biological replicates (n = 7?8)