ZFIN Image: Rocha et al., 2021, Fig. 3

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Figure Caption

Fig. 3 Cdx4 regulates posterior expression of foxd3. A. Analysis of Cdx4 and Foxd3 binding near the foxd3 locus. B-E. Dorsal view of 15 hpf WT or cdx4^ch107+/− heterozygous embryo shows foxd3 RNA transcripts detected by HCR in the dorsal spinal cord and tailbud (B) counterstained with DAPI (C). D. Merged image of B and C; arrow indicates tailbud, arrowhead labels neural tube. Scale bar = 100 μm. E. Magnified view of tailbud region from D; scale bar = 50 μm. F–I. Dorsal view of 15 hpf cdx4^ch107−/- mutant embryo shows foxd3 RNA transcripts detected by HCR in the dorsal spinal cord and lack of expression in the tailbud (F) counterstained with DAPI (G). H. Merged image of F and G; arrow indicates tailbud, arrowhead labels neural tube. Scale bar = 100 μm. I. Magnified view of tailbud region from H; scale bar = 50 μm.

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Acknowledgments

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Reprinted from Developmental Biology, 480, Rocha, M., Kushkowski, E., Schnirman, R., Booth, C., Singh, N., Beadell, A., Prince, V.E., Zebrafish Cdx4 regulates neural crest cell specification and migratory behaviors in the posterior body, 25-38, Copyright (2021) with permission from Elsevier. Full text @ Dev. Biol.