Fig. 1

Fig. 1 Zebrafish cdx4 is expressed in NC cells. A-C. Lateral views of a 15 hpf embryo; A’-C’ are views of a single 5 ​μM optical section of a hand-cut transverse section from the trunk. A. cdx4 mRNA detected in the tailbud and spinal cord by HCR. The anterior limit of expression corresponds to the hindbrain/spinal cord boundary (arrow). A’. cdx4 expression in a 5 ​μM optical section from the trunk (line in A); n.t. indicates the neural tube, s. indicates the somite. B. foxd3 mRNA detected in NC cells along the AP axis, the posterior somites, and the tailbud. B’. foxd3 expression in the 5 ​μm optical section (line in B); arrowhead indicates foxd3 expression in the somitic mesoderm. C. co-detection of cdx4 and foxd3 at the dorsal neural tube, scale bar ​= ​100 ​μm. C’. cdx4 and foxd3 co-expression in the premigratory trunk NC cells in the 5 ​μm optical section (line in C); scale bar ​= ​25 ​μm. D-F. Dorsal view of the tailbud of a 12 hpf embryo. D. cdx4 mRNA transcripts in the tailbud detected by HCR. E. foxd3 mRNA transcripts in the tailbud detected by HCR. F. Merged image of A and B reveals co-expression of cdx4 and foxd3 transcripts at the tailbud. Dashed lines demarcate the outline of the neural plate border (inner line) and the yolk; scale bar ​= ​100 ​μm. G, H. Lateral views of a 12 hpf embryo, scale bar ​= ​100 ​μm; G cdx4 mRNA detected in the tailbud and spinal cord by HCR. The anterior limit of expression is indicated (arrow). H. foxd3 mRNA detected in NC cells along the entire AP axis and in the tailbud (arrowhead) by HCR. I. Dorsal view (as indicated by arrowhead in H) of a high magnification (40x objective) 0.75 ​μm single optical section of the tailbud of a 12 hpf embryo, colabeled with DAPI (grey), showing that cdx4 and foxd3 transcripts colocalize to single cells, scale bar ​= ​25 ​μm.