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Fig. 9
Gene expression patterns of the zebrafish kcnt genes. Whole‐mount in situ hybridization of zebrafish embryos at stages 12hpf (A, E, I, M, Q, U, Y, CC), 24hpf (B, F, J, N, R, V, Z, DD), 48hpf (C, G, K, O, S, W, AA, EE), and 72hpf (D, H, L, P, T, X, BB, FF). Anterior is to the left in all the whole‐mount images, and dorsal is to the top in all transverse sections, except panel B′ and B″. Each embryo was imaged laterally and dorsally, respectively. A‐D, Lateral view of gene expression of kcnt1a. E‐H, Dorsal view of gene expression of kcnt1a. I‐L. Lateral view of gene expression of kcnt1b. M‐P, Dorsal view of gene expression of kcnt1b. Q‐T, Lateral view of gene expression of kcnt2a. U‐X, Dorsal view of gene expression of kcnt2a. Y‐BB, Lateral view of gene expression of kcnt2b. CC‐FF, Dorsal view of gene expression of kcnt2b. The dashed lines indicate the approximate positions of sections. The letters below or around the dashed lines correspond to the panels. Scale bars are added on the top row of images; 250 μm for whole mount images and 50 μm for tissue sections. e, eye; hb, hindbrain; hypo, hypothalamus; kv, Kupffer's vesicle; n, neural tube; nn, neural nucleus; nt, notochord; ol, olfactory placodes/organ; op, optic vesicle; ov, otic vesicle; pal, pallium; ptc,, posterior tuberculum; rhb, rohon beard neurons; subp, subpallium; tb, tailbud; th, thalamus; tgm, tegmentum