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Fig. 5
Knockout of lrpap1 in zebrafish leads to increased apoptosis in the eye. (A) AO was used to quantify apoptosis in the eyes of 24 hpf embryos: green fluorescence, as highlighted using white triangles. (B) TUNEL staining was also used to investigate ocular apoptosis in adult zebrafish. Apoptosis is highlighted by yellow triangles, particularly in the choroidal tissue (magnified image). The green circles highlight apoptotic cells in the sclera. The scale bars refer to 200 ?m. WT, wild-type. MU, lrpap1 homozygous mutant. dpf, days post-fertilization
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