FIGURE 2

FIGURE 2

Iron supplementation could improve the survival activity of ZFL cells under hypoxia stress, but iron chelation and inhibition of ferroptosis could not. (A–C) Survival rate of ZFL cells analyzed with PrestoBlue™ HS Cell Viability Regent after treated with a series of concentrations of FAC (A), DFO (B) and Fer-1 (C) under hypoxic stress from one to 4 days. Hypoxia 0 mM or hypoxia 0 µM was used as a control group for significance analysis. (D) The microscope analysis of morphology changes of ZFL cells under normoxia or treated with FAC (2.5 mM), DFO (10 µM) and Fer-1 (2.5 µM) under hypoxic stress for 3 days. Error bars, mean ± s.d., n = 3 (biological replicates).