FIGURE 2

FIGURE 2

Hair cell quantification following treatment with COVID-19 therapies. Hair cells were counted from images represented in Figure 1. Controls (“0,” white bars) received vehicle, which was methanol for dexamethasone-treated fish and DMSO for all other drugs. Dexamethasone was used as a negative control. Hair cells were counted in five neuromasts per fish and summed to arrive at one value per animal. Data were analyzed by one-way ANOVA followed by Bonferroni-corrected post hoc tests. One-way ANOVA results and sample sizes are as follows: Dexamethasone F₍₆,₆₃₎ = 3.53, p = 0.0045, N = 10; Remdesivir F₍₆,₇₃₎ = 4.054, p = 0.0015, N = 10–20; Azithromycin F₍₆,₅₅₎ = 1.28, p = 0.281, N = 6–10; Imatinib F₍₆,₆₃₎ = 188.0, p < 0.0001, N = 6–20; Lopinavir F₍₆,₇₂₎ = 80.15, p < 0.0001, N = 9–20; Ritonavir F₍₆,₇₅₎ = 27.99, p < 0.0001, N = 11–13; Ivermectin F₍₃,₃₆₎ = 8.59, p = 0.0002, N = 10. Asterisks indicate significance differences from vehicle controls. *p < 0.05, ****p < 0.0001. Note that the imatinib and lopinavir experiments were run concurrently and therefore shared control animals. Data are represented as mean ± 1 s.d. and black dots represent individual fish.