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Fig. 6
IPL ribbon synapses of bipolar cells are preserved in hyperglycemic larvae. (A) Transverse retinal sections from 5 dpf control (top) and hyperglycemic (bottom) larvae were double immunostained with fluorescently labeled antibodies specific for the rod bipolar cell marker PKC? (cyan; left panels) or ribeye a (red; middle panels); also shown is the overlay of PKC? and ribeye a labeling (merge; right panels). Maximal intensity projections are shown, and the relative positions of the OPL, INL, and IPL are indicated. Scale bar, 5 µm. The expression pattern of PKC?-labeled rod bipolar cell neurons, synaptic ribbons in the IPL layer, and the number of ribbon synapses in rod bipolar cells are normal in 5 dpf hyperglycemic larvae. (B) Quantitative analyses of IHC for colocalization of PKC? and ribeye a are indicated by box-and-whisker plots. Boxes indicate median values, and whiskers indicate 5th?95th percentile values of pooled data from 56 larvae. The data represent the total ribeye a labeling in PKC?-labeled terminals (p > 0.05). Abbreviations used: Dpf, days post-fertilization; PKC?, protein kinase C alpha; SEM, standard error of the mean.