|
Fig. 2
TFAP2B transcript and protein expression analysis. (A) qRT-PCR analysis of TFAP2B transcript levels of WT, c.706 C > T, c.898 C > T, c.1144 C > T, and exon 4 deletion expression constructs show no statistically significant difference among the samples normalized to GAPDH and b-Actin. (B) Western blot analysis of all tested variants shows absence of protein products for the syndromic craniosynostosis variant (c.1144 C > T) and PIPO variant (del Exon 4). Protein products are still detectable for the Char syndrome variants (c.706 C > T and c.898 C > T). A Myc-tag antibody was used to visualize TFAP2B protein levels.