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Figure 4—figure supplement 2.

ID: ZDB-IMAGE-220726-39
Publication: Satou et al., 2022 - A viral toolbox for conditional and transneuronal gene expression in zebrafish
All Figures: Figures for Satou et al., 2022

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Figure Caption

Figure 4—figure supplement 2. Transneuronal tracing using pseudotyped rabies virus from <italic toggle='yes'>vglut1</italic> +neurons in Dp in adult zebrafish.

(A) Co-injection of EnvA-RVΔG-GFP and HSV1[UAS:TVA-mCherry] into Dp of Tg[vglut1:Gal4] fish in the absence of glycoprotein. Coronal section through the injected telencephalic hemisphere at the level of Dp. Area outlined by dashed rectangle is enlarged below and red channel is enhanced. Co-expression of GFP (green) and mCherry (magenta) indicates starter cells. (B) Same as in (A) but with trans-complementation of zoSADG in starter neurons by co-injection of HSV1[UAS:zoSADG]. Left: coronal section through the injected telencephalic hemisphere. Right: coronal section through the ipsilateral olfactory bulb. Expression of GFP only (green) indicates transneuronally labeled neurons.

Acknowledgments

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