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Fig. 1 Dynamic DNA methylation during HSPC generation. (A) Flowchart of sorting and whole genome bisulfite sequencing. EC, endothelial cell; HEC, hemogenic endothelial cell; HSPC, hematopoietic stem and progenitor cell; WGBS, whole genome bisulfite sequencing. (B) Global DNA methylation levels in EC, HEC, HSPC and fraction of CpGs displaying high (>0.8), intermediate (≥0.2 and <0.8), low (>0 and <0.2) and no (=0) 5mC. n=3 replicates. (C) Heatmap displays differentially methylated regions (DMRs) in EC, HEC and HSPC. Right column shows functional enrichment of DMR-related genes by GREAT analysis. The x-axis represents the negative log of the P-values of the enrichment of the corresponding GO terms. C1-C6, Clusters 1-6. (D) The percentage of hyper-DMRs and hypo-DMRs between each two consecutive stages: EC versus HEC and HEC versus HSPC. (E) GO enrichment of DMRs by GREAT analysis between each two consecutive stages: EC versus HEC and HEC versus HSPC. The x-axis represents the negative log of the P-values of the enrichment of the corresponding GO terms.