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Figure 5
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Figure 5
Figure 5. Biosynthesis of amino acids and aminoacyl-tRNA were enhanced in slc38a9−/− embryos. (A) Heatmaps of transcripts in the biosynthesis of amino acids’ enrichment. (B) Heatmaps of transcripts in aminoacyl-tRNA synthetases’ enrichment. (C) Most free amino acids (FAAs) increased in slc38a9−/− mutants. The contents of most FAAs (Leu, Met, Ile, Val, Lys, etc.) increased except for Pro, Glu, and Asp. The profile of FAAs was measured using the automated amino acid analyzer. (D) The relative FPKM of major aminotransferases in WT and slc38a9 mutant zebrafish embryos (60 hpf). Data shown are mean ± SEM, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.005.
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