Fig. 4
Histological distribution and enzymatic activity of Zebrafish- α-GAL protein in the GLA mutant zebrafish. (A) Scanned digital images show α-GAL antibody and periodic acid Schiff staining. Note that PAS staining shows dilated capillary loops and thinner Bowman's space (yellow arrowheads) in mutant compared to wild type animals. (B) Quantification of immunohistochemical analysis of sections from wild-type and mutant kidneys. Signal intensity is significantly higher in wild type compared to mutant tissues (Mann-Whitney