Fig. 2

Fig. 2

Kidney tissue distribution and enzyme activity in wild type zebrafish. (A) Immunohistochemistry (IHC) staining for Zebrafish α-GAL in wild-type adult zebrafish kidney. The expression pattern is similar in both genders. The protein was abundantly detectable in the cytoplasm of renal tubule cells, and to a lower extent in the glomerulus. Hematoxylin was used as counterstain. The lower panel shows the three renal structures, as stained with Periodic Acid Schiff (PAS). Scale bar (in black) = 50um. (B) Wild-type zebrafish α-GAL activity as evaluated in the whole embryo, larvae, and juvenile whole body tissue lysates and in adult male and female kidney tissue lysates (Kruskal-Wallis, P ≤ 0.05). Results of the Dunn post hoc test show a significant difference (*) between juvenile and larvae (P < 0.05) and juvenile and adult female specimens (P < 0.05). The violin plot represents these results within the 95th percentile. G = glomerulus, PT = proximal tubule, DT = distal tubule. Validation of the antibody is provided in Sup.4.