FIGURE 2

FIGURE 2

Transcriptome profiling and functional annotation of the telencephalon during early wound healing (1 dpl), early proliferative (3 dpl) and differentiation (14 dpl) stages of zebrafish brain regeneration. (A) Heatmaps of log₂ fold changes of selected genes across three stages of brain regeneration. Each column represents a time point and each row shows a single gene. The scale bar shows red for Up, blue for Down, yellow for weak regulation (FC < 1.5 in either direction) or statistically non-significant (Benjamini–Hochberg adjusted p-value (FDR) > 0.1). (B–D) GO-BP terms enriched at 1 dpl (B), 3 dpl (C) and 14 dpl (D) by using all DEGs. DAVID was used to show the most significantly enriched GO-BP terms. All DEGs (1 dpl: 6,123, 3 dpl: 4,662, 14 dpl: 1954) were used for the analyses. The heatmap scale shows log₁₀ of the ease p-values for the most significantly enriched GO terms. (E) Relative expression levels of genes that are Down or Up at different stages of regeneration. bdnf is Down at 3 dpl and 14 dpl, while syt2a and epha6 are Down at all stages. capgb is Up at all stages, while gfap is Up at 1 dpl and 3 dpl. Statistical significance was evaluated using unpaired t-test. *p < 0.05, **p < 0.01 and ***p < 0.001. ns: non-significant. Error bars represent ±standard error of mean (SEM, n = 3). Up: upregulated, Down: downregulated, dpl: days post-lesion, DAVID: database for Annotation, Visualization and Integrated Discovery, GO: Gene Ontology, BP: Biological Process.