Fig. 2
Loss of aldh2.1 led to increased angiogenesis in the retinal vasculature of zebrafish larvae and adults. A: Representative confocal images of hyaloid vasculature in zebrafish larvae at 120 hpf displayed increased branching. Red scale bar: 20 ?m, red arrows: branch points as counted for quantification. B: Representative confocal images of adult zebrafish retinae showed increased angiogenesis. White Scale Bar: 350 ?m, red rectangle: high-density subdivision, blue rectangle 350 ?m square, red arrows: branch points as counted for quantification. C: Quantification of larval hyaloid vasculature showed increased numbers of branch points in aldh2.1?/? mutants, n = 22?24 eyes per group. D: Quantification of retinal vasculature showed increased numbers of branch points in aldh2.1?/? adults, n = 16?17 350 ?m squares per group, statistical analysis was done via Student's - test, *p < 0.05, ***p < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)