ZFIN Image: Wu et al., 2021, F

Image

Figure Caption

F Galectin‐9 is essential for AG‐induced expression of MMPs

A

Immunoblots of cell lysates of THP‐1 cells stably transfected with scrambled shRNA or shRNA targeting galectin‐9.

B–D

qPCR analysis of Mmps including Mmp9 (B), Mmp10 (C), and Mmp12 (D) mRNA from control or Galectin‐9 knockdown THP‐1 cells stimulated with AG (1 μg/ml) for indicated times.

E

Diagram showing the gRNA‐targeting genome sites.

F

Identification of galectin‐9 KO mice with PCR.

Data information: Data in (B–D) are means ± SD averaged from n = 3 independent experiments performed with technical triplicates, and each symbol represents the mean of technical triplicates. Data in (A, F) are representative of at least n = 2 independent experiments. Two‐way ANOVA followed by Tukey's post hoc test (B–D) was used for statistical analysis, respectively. ns, not significant; *P < 0.05; ****P < 0.0001.

Source data are available online for this figure.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO Rep.