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Figure 4
Main EMT hallmarks E-cadherin and Vimentin in liver tissues from twist1a+/kras+ double transgenic zebrafish. (A) Immunohistochemical staining micrograph showing E-cadherin and Vimentin-positive cells in liver cross-sections of wild-type, twist1a+, kras+, and twist1a+/kras+ zebrafish. (B) Quantification of the percentage of E-cadherin and Vimentin-positive cells for all fields following staining at 4 wpi using ImageJ. Scale bar: 50 μm. Student’s t-tests were used to assess differences between variables: * p < 0.05, ** p < 0.01, *** p < 0.001.
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