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Fig. 4
AKT activation promotes the nucleus localization of RNF12.
A Immunofluorescence and 4, 6-diamidino-2-phenylindole (DAPI) staining of HeLa cells stably transfected with RNF12 WT, S215A, S347A/T349A, or S215A/S347A plasmids. Left panel: Number of cells (A) were analyzed according to the following subgroups: cytoplasm, nuclear, or at both locations (both). B Immunofluorescence and DAPI staining of MDA-MB-231 cells treated with IGF-1 (200 ng/mL) and MK2206 (5 μM). C Immunofluorescence and DAPI staining of HeLa cells transfected with Flag-RNF12 and GFP-SMAD7, treated with DMSO or MK2206 (5 μM) for 8 h. D Western blotting experiments relevant with C.
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