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Fig. 5 The coordination of the cell cycle state and pancreatic fate propensity involves Shh signaling. (A) The shha mRNA but not bmpMO or the dominant-negative Bmpr rescued the reduction of pancreatic endocrine cells caused by overexpression of geminin or Cdk2. The shhaMO rescued the expansion of pancreatic endocrine cells caused by gemMO or Cdkn1c overexpression. (B) The results were confirmed by the statistics (n = 5). (Scale bar, 50 μm.) Data are expressed as mean ± SD; P value is calculated using Student’s t test.
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