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Fig. 5 Riboflavin supplementation suppresses oxidative stress in etfa+/− mutant livers. Adult zebrafish were exposed to riboflavin, as in Fig. 4. (A) Relative ROS, (B) GSH levels, and (C) MDA levels in wild-type and etfa+/− livers from untreated or riboflavin-treated adult zebrafish were measured (see Materials and Methods) (n = 6 per group). Relative mRNA expression of (D) NADPH oxidases and (E) oxidative stress-responding genes was measured (see Materials and Methods; three livers were used to make normalized RNA; *P < 0.05, **P < 0.005). Abbreviations: gstp1/2, glutathione S transferase pi 1 and 2; ho-1a, heme oxygenase 1a; nrf2, nuclear factor erythroid 2-related factor 2; prdx4, peroxiredoxin 4.