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Figure 4
Effect of neomycin concentration and treatment intervals on hair cell death. Boxplots represent the number of hair cells per neuromast for both the 6-h and 12-h treatment timelines. (A) Total (GFP+) and (B) functional (FM+) hair cells at imaging timepoints I1 and I3. The Kruskal–Wallis ANOVA with Dunn post-test results are provided in Table S3. (C) Comparison of the 6-h and 12-h timelines in terms of the number of GFP+ and FM+ hair cells remaining after treatment at imaging timepoint I3 on 4 dpf. There is no significant difference in the hair cell counts between the two treatment timelines (Kruskal-Wallis ANOVA with Dunn post-test, α = 0.05; Table S4). Significance levels are as follows: *** = p < 0.001, ** = p < 0.01, * = p < 0.05, ns = not significant.