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Fig 5
A ATCC 19977, D436H mutant, and D436H:leuSD complement strains were grown in Sauton media with 59 μg/mL norvaline (top left), 66 μg/mL leucine (top right), 59 μg/mL valine (lower left), or 66 μg/mL isoleucine (lower right). Data shown is from three biological replicates, one-way ANOVA with Tukey’s multiple comparisons test. ****, p < 0.0001. B D436H mutant grown in Sauton media with 59 μg/mL norvaline and a range of leucine (left), isoleucine (middle), or valine (right). D436H mutant grown in 0 μg/mL norvaline and 0 μg/mL BCAA represents growth control. D436H mutant grown in 59 μg/mL norvaline and 0 μg/mL BCAA represents inhibited growth control. BCAAs added to cultures from 33 μg/mL to 262 μg/mL (Leu/Ile) or 29 μg/mL to 234 μg/mL (Val). Data shown is mean ± SD from three biological replicates. Groups compared to inhibited growth control with one-way ANOVA with Dunnett’s multiple comparisons test. ***, p = 0.0009; ****, p ≤ 0.0001.