Figure 4

Figure 4

Glucose-injected larvae have increased lipid accumulation following a high fat diet. (A) Schematic of the high fat feeding challenge assay to measure lipid accumulation in glucose-injected larvae. (B) Bright field images of 6 dpf Oil Red O-stained larvae, demonstrating darker vascular staining in glucose-injected larvae. Box indicates location of inset, arrowheads indicate stained intersegmental vessels in inset, asterisk indicates intestinal lumen which was excluded from analysis. (C) Quantification of lipid accumulation in 5 dpf glucose-injected larvae. (D) Quantification of lipid accumulation in 6 dpf glucose-injected larvae challenged with a high fat diet from 5 dpf. (E) Quantification of lipid accumulation in 7 dpf glucose-injected larvae challenged with a high fat diet from 5 dpf. Statistical testing by t test. Data are representative of 2 biological replicates.