ZFIN Image: Lepanto et al., 2021, Fig. 5.

Image

Figure Caption

Fig. 5.

Different cell morphologies observed in fabp4a(-2.7):EGFPcaax larvae. (A) fabp4a(-2.7):EGFPcaax larvae of 21 dpf were labeled with LipidTOX-Red and imaged in vivo through confocal microscopy. Images are 3D projections of confocal stacks, to show different cell morphologies found in these larvae. (B) Images of fabp4a(-2.7):EGFPcaax; kdlr:mCherry larvae of SL 8 mm (19 dpf). Note labeled cells in the AVAT depot with cytoplasmic projections which lay in close apposition to blood vessels. Images are 3D projections or sections as indicated. (C,D) High magnification confocal sections of EGFP+ cells in larvae stained with Nile Red (C) or LipidTOX-Red (D). The cytoplasmic inclusions observed in transmitted light in these cells showed a polar lipid profile (evidenced by Nile Red staining, C) and low signal intensity of LipidTOX-Red (D). Scale bars: A: 20 ?m; B: 20 ?m; C,D: 10 ?m.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biol. Open