ZFIN Image: Feng et al., 2021, Figure 4

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Figure Caption

Figure 4

Ergocalciferol and panthenol facilitate HSPCs expansion in a dosage-dependent manner. (A) Schematic diagram of concentration gradient using ergocalciferol and panthenol. (B) WISH of cmyb with different concentration (5, 10, 20 ?M). Blue arrowheads indicate cmyb⁺ signals. (C) WISH of lyz (upper) and gata1 (bottom) after treating with ergocalciferol and panthenol using 20 ?M. (D,E) Quantification of lyz⁺ (left) and gata1⁺ (right) signals areas in the CHT region. (Pixels, lyz⁺ signals; DMSO, 4980 � 467; Ergocalciferol, 8678 � 617; Panthenol, 7524 � 468; gata1⁺ signals; DMSO, 8525 � 768; Ergocalciferol, 16,350 � 1816; Panthenol, 13,790 � 1252). Scale bar, 50 ?m; Mean � SEM, n = 8; ** p < 0.01, *** p <0.001.

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