Fig 4

Fig 4

(A-D) Confocal projections of transgenic Tg(fli1a:GFP)^y1 embryos at 28 hpf. Wild type embryo is on the top left panel (A), prmt5 mutant embryos were not injected (B) or injected with either prmt5WT mRNA (C) or the mutant form prmt5MUT mRNA (D). Scale bar 100 μm. (E, F) Confocal projections of transgenic Tg(fli1a:GFP)y1 embryos treated with either DMSO (E) or EPZ 20 μM (F) from 9 hpf to 28 hpf. (G, H) Average ISVs length in μm (G) and average number of endothelial cells per ISVs (H) of embryos. Data were from 3 independent experiments with at least 3 animals per condition. Kruskal-Wallis test (G) and One-way ANOVA (H) were performed. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. (I, J) Relative expression of prmt5 (I) or etv2 (J) mRNA by RT-qPCR on 28 hpf wild type and prmt5 mutant embryos from 3 independent experiments with at least 6 animals per condition. T-test was performed. ***P<0.001. (K) Log2 fold change expression relative to wt embryos of cdh5, fli1b, agtr2, esama and fli1a mRNAs by RT-qPCR on 28 hpf prmt5 mutant embryos not injected or injected by either prmt5WT or prmt5MUT mRNAs, from at least 2 independent experiments with a minimum of 6 animals per condition. Two-way ANOVA was performed. * P<0.05. **P<0.01.